Recombinant Human Interferon-alpha2b, Ecoli. (rHuIFN-alpha2b,Ecoli.)
英文全名
Recombinant Human Interferon-alpha2b, Ecoli. (rHuIFN-alpha2b,Ecoli.)英文又名
卵白布局
氨基酸序列
MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKEAccession
Gene ID
产物英文概述
IFN-alphas are proteins secreted by leukocyte. They are mainly involved in innate immune response against viral infection. The IFN-alpha family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.来历
Escherichia coli.份子量
Approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 166 amino acids.生物活性
Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 × 108 IU/mg.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.内毒素
Less than 1 EU/ug of rHuIFN-α2b, Ecoli. as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
纯度
> 96 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
