Recombinant Human Interleukin-19 (rHuIL-19)
英文全名
Recombinant Human Interleukin-19 (rHuIL-19)英文又名
Melanoma Differentiation-associated Protein-like Protein, NG.1卵白布局
氨基酸序列
LRRCLISTDM HHIEESFQEI KRAIQAKDTF PNVTILSTLE TLQIIKPLDV CCVTKNLLAF YVDRVFKDHQ EPNPKILRKI SSIANSFLYM QKTLRQCQEQ RQCHCRQEAT NATRVIHDNY DQLEVHAAAI KSLGELDVFL AWINKNHEVM SSAAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.生物活性
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 1.5 ng/ml, corresponding to a specific activity of > 6.7 × 105 IU/mg.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.内毒素
Less than 1 EU/ug of rHuIL-19 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100 mM HAC to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Chang C, Magracheva E, Kozlov S, et al. 2003. J Biol Chem, 278: 3308-13.2. Gallagher G, Dickensheets H, Eskdale J, et al. 2000. Genes Immun, 1: 442-50.3. Liao YC, Liang WG, Chen FW, et al. 2002. J Immunol, 169: 4288-97.纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
