Recombinant Human Interleukin-7 GMP (rHuIL-7)
英文全名
Recombinant Human Interleukin-7 GMP (rHuIL-7)英文又名
LP-1, pre-B cell factor卵白布局
氨基酸序列
DCDIEGKDGK QYESVLMVSI DQLLDSMKEI GSNCLNNEFN FFKRHICDAN KEGMFLFRAA RKLRQFLKMN STGDFDLHLL KVSEGTTILL NCTGQVKGRK PAALGEAQPT KSLEENKSLK EQKKLNDLCF LKRLLQEIKT CWNKILMGTK EHAccession
Gene ID
产物英文概述
Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Murine IL-7 has approximately 65 % amino acid sequence identity with human IL-7 and both proteins exhibit cross-speciesactivity. IL-7 as an immunotherapy agent has been examinedin many human clinical trials for various malignancies and during HIV infection.来历
Escherichia coli.份子量
Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.生物活性
Assay #1:Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine 2E8 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL) is 0.1-0.5 ng/mL. The specific activity of Recombinant Human IL-7 is approximately 2.0 × 108 units/mg, which is calibrated against human IL-7 WHO Standard (NIBSC code: 90/530).
表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.内毒素
Less than 0.01 EU/ug of rHuIL-7 GMP as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Goodwin RG, Lupton S, Schmierer A, et al. 1989. Proc Natl Acad Sci U S A. 86:302-6.2. Sutherland GR, Baker E, Fernandez KE, et al. 1989. Hum Genet. 82:371-2.3. Lupton SD, Gimpel S, Jerzy R, et al. 1990. J Immunol. 144:3592-601.4. Noguchi M, Nakamura Y, Russell SM, et al. 1993. Science. 262:1877-80.5. Fry TJ, Mackall CL. 2002. Blood. 99:3892-904.6. Fry TJ, Mackall CL. 2002. J Hematother Stem Cell Res. 11:803-7.纯度
> 98 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
