Recombinant Human Macrophage Inflammatory Protein-4/CCL18 (rHuMIP-4/CCL18)
英文全名
Recombinant Human Macrophage Inflammatory Protein-4/CCL18 (rHuMIP-4/CCL18)英文又名
AMAC-1, PARC, DC-CK1卵白布局
氨基酸序列
AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNAAccession
Gene ID
产物英文概述
Human CCL18 is encoded by the CCL18 gene located on the chromosome 17. As also named MIP-4, it shares 61 % sequence identity to human MIP-1alpha. CCL18 is mainly expressed by lung and some lymphoid tissues like lymph nodes express CCL18 at low level. It is chemotactic for both activated (CD3+) T cells and nonactivated (CD14-) lymphocytes, but not for monocytes or granulocytes. Involved in B-cell migration into B-cell follicles in lymph nodes. CCL18 plays a role in both humoral and cell-mediated immunity responses.来历
Escherichia coli.份子量
Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.生物活性
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 1.0-10 ng/ml.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.内毒素
Less than 1 EU/ug of rHuMIP-4/CCL18 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
纯度
> 97 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
