Recombinant Human Macrophage Migration Inhibitory Factor, His (rHuMIF,His)
英文全名
Recombinant Human Macrophage Migration Inhibitory Factor, His (rHuMIF,His)英文又名
GLF, L-dopachrome Isomerase, Phenylpyruvate Tautomerase卵白布局
氨基酸序列
MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFALEHHH HHHAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 13.5 kDa, a single non-glycosylated polypeptide chain containing 117 amino acids, with 6 × His at the C-terminus.生物活性
Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.表面
Sterile Filtered White lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.内毒素
Less than 1 EU/ug of rHuMIF, His as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Edwards KM, Tomfohr LM, Mills PJ, et al. 2011. Sleep, 34: 161-3.2. Delaloye J, De Bruin IJ, Darling KE, et al. 2012. Cytokine, 3. Leu RW, Woodson PD, Whitley SB. 1977. J Reticuloendothel Soc, 22: 329-40.4. Landolfo S. 1977. G Batteriol Virol Immunol, 70: 137-43.5. Baugh JA, Chitnis S, Donnelly SC, et al. 2002. Genes Immun, 3: 170-6.纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
