Recombinant Human Nephroblastoma-overexpressed Gene Protein Homolog (rHuNOV)
英文全名
Recombinant Human Nephroblastoma-overexpressed Gene Protein Homolog (rHuNOV)英文又名
卵白布局
氨基酸序列
MQVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK MAccession
Gene ID
产物英文概述
来历
份子量
Approximately 36.2 kDa, a single non-glycosylated polypeptide chain containing 331 amino acids.生物活性
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 ug/ml, corresponding to a specific activity of > 1000 IU/mg.表面
Sterile Filtered White lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.6, 150 mM NaCl.内毒素
Less than 0.1 EU/ug of rHuNOV as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
