Recombinant Human Neutrophil Activating Protein-2/CXCL7 (rHuNAP-2/CXCL7)
英文全名
Recombinant Human Neutrophil Activating Protein-2/CXCL7 (rHuNAP-2/CXCL7)英文又名
CXCL7, LDGF, MDGF, Small-inducible cytokine B7卵白布局
氨基酸序列
AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KDGRKICLDP DAPRIKKIVQ KKLAGDESADAccession
Gene ID
产物英文概述
Neutrophil activating protein-2 also named CXCL7 is an isoform of Beta-Thromboglobulin or Pro-Platelet basic protein. It belongs to the CXC chemokine family and is released in large amounts from platelets following their activation. CXCL7 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. Recombinant human CXCL7 contains 70 amino acids which is a single non-glycosylated polypeptide chain. In addition, The human CXCL7 shares 53 % and 58 % a.a. sequence identity with mouse and rat CXCL7.来历
Escherichia coli.份子量
Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.生物活性
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 1.0-10.0 ng/ml.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.内毒素
Less than 1 EU/ug of rHuNAP-2/CXCL7 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
纯度
> 97 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
