Recombinant Human Noggin, Insect Cells Derived (rHuNoggin,InsectCell)
英文全名
Recombinant Human Noggin, Insect Cells Derived (rHuNoggin,InsectCell)英文又名
卵白布局
氨基酸序列
QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSCAccession
Gene ID
产物英文概述
来历
份子量
Approximately 23.0 kDa on SDS-PAGE under reducing conditions, containing 205 amino acids, and a molecular mass about 47.9 kDa homodimer under non-reducing conditions (Molecular size on SDS-PAGE will appear at approximately 50-80 kDa).生物活性
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.04‑0.2 ug/mL in the presence of 50 ng/mL of Recombinant Human BMP‑4.表面
Sterile Filtered White lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4, 5% trehalose, 0.02%Tween-20.内毒素
Less than 0.1 EU/ug of rHuNoggin, Insect Cell as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
