Recombinant Human PCT/Procalcitonin (rHuPCT/Procalcitonin)
英文全名
Recombinant Human PCT/Procalcitonin (rHuPCT/Procalcitonin)英文又名
卵白布局
氨基酸序列
Accession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
生物活性
No data available.表面
Sterile Filtered White lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl.内毒素
Less than 0.1 EU/ug of rHuPCT/Procalcitonin as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- Refer to lot specific COA for the Use by Date when stored at ≤ -20 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
Procalcitonin (PCT) belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). PCT is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. PCT is produced by parafollicular cells (C cells) of the thyroid and by the neuroendocrine cells of the lung and the intestine. But its level is related to the severity of bacterial sepsis, it is considered to be one of the earliest and most specific markers of sepsis.纯度
> 98 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
