Recombinant Human Platelet-derived Growth Factor-AA (rHuPDGF-AA)
英文全名
Recombinant Human Platelet-derived Growth Factor-AA (rHuPDGF-AA)英文又名
卵白布局
氨基酸序列
Accession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
生物活性
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 50-200 ng/mL in a fluorometric assay using the redox sensitive dye.表面
Sterile Filtered White lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 6.0.内毒素
Less than 0.1 EU/ug of rHuPDGF-AA as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
纯度
> 95 % by SDS-PAGE.SDS-PAGE电泳
