Recombinant Human Secreted Protein Acidic and Rich in Cysteine, His (rHuSPARC,His)
英文全名
Recombinant Human Secreted Protein Acidic and Rich in Cysteine, His (rHuSPARC,His)英文又名
Basement-membrane Protein 40, BM-40, Osteonectin, ON卵白布局
氨基酸序列
MSYYHHHHHH DYDIPTTENL YFQGAMGSAP QQEALPDETE VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE NPCQNHHCKH GKVCELDENN TPMCVCQDPT SCPAPIGEFE KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT EKQKLRVKKI HENEKRLEAG DHPVELLARD FEKNYNMYIF PVHWQFGQLD QHPIDGYLSH TELAPLRAPL IPMEHCTTRF FETCDLDNDK YIALDEWAGC FGIKQKDIDK DLVIAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 36.1 kDa, a single non-glycosylated polypeptide chain containing 314 amino acids, with expression vector sequence (containing 6 × His tag).生物活性
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 ug/mL, corresponding to a specific activity of > 333 IU/mg.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.内毒素
Less than 1 EU/ug of rHuSPARC, His as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Sage H, Decker J, Funk S, et al. 1989. J Mol Cell Cardiol, 21 Suppl 1: 13-22.2. Kanauchi M, Nishioka M, Dohi K. 2000. Diabetologia, 43: 1076-7.3. Lau CP, Poon RT, Cheung ST, et al. 2006. J Pathol, 210: 459-68.4. Rodriguez-Jimenez FJ, Caldes T, Iniesta P, et al. 2007. Oncol Rep, 17: 1301-7.5. Wong SY, Crowley D, Bronson RT, et al. 2008. Clin Exp Metastasis, 25: 109-18纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
