Recombinant Human Stromal-Cell Derived Factor-1 alpha/CXCL12 alpha (rHuSDF-1alpha/CXCL12alpha)
英文全名
Recombinant Human Stromal-Cell Derived Factor-1 alpha/CXCL12 alpha (rHuSDF-1alpha/CXCL12alpha)英文又名
SDF-1 alpha, hSDF-1 alpha, IRH, hIRH, PBSF卵白布局
氨基酸序列
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKAccession
Gene ID
产物英文概述
CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the C-terminal tail. And all SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to the canonical sequence SDF-1β, SDF-1alpha is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression.来历
Escherichia coli.份子量
Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.生物活性
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/ml.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB pH 7.0, 130 mM NaCl.内毒素
Less than 1 EU/ug of rHuSDF-1α/CXCL12α as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
纯度
> 97 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
