Recombinant Human TNF-related Weak Inducer of Apoptosis Receptor/TNFRSF12A (rHuTWEAKReceptor/TNFRSF12A)
英文全名
Recombinant Human TNF-related Weak Inducer of Apoptosis Receptor/TNFRSF12A (rHuTWEAKReceptor/TNFRSF12A)英文又名
TNFRSF12A, FGF-inducible 14, CD266卵白布局
氨基酸序列
EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWPAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 5.6 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids.生物活性
Fully biologically active when compared to standard. The ED50 as determined by inhibiting TWEAK-dependent proliferation of human umbilical vein endothelial cells (HUVEC) is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 104 IU/mg, in the presence of 15 ng/ml of rHuTWEAK.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.内毒素
Less than 1 EU/ug of rHuTWEAK Receptor as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Gaudineau B, Fougere M, Guaddachi F, et al. 2012. J Cell Sci, 125: 4475-86.2. Abend JR, Uldrick T, Ziegelbauer JM. 2010. J Virol, 84: 12139-51.3. Kang S, Yang C, Luo R. 2008. Biochem Biophys Res Commun, 372: 629-33.4. Wiley SRandWinkles JA. 2003. Cytokine Growth Factor Rev, 14: 241-9.纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
