Recombinant Human Ubiquitin-conjugating Enzyme E2 B, His (rHuUBE2B,His)
英文全名
Recombinant Human Ubiquitin-conjugating Enzyme E2 B, His (rHuUBE2B,His)英文又名
HR6B, Ubiquitin Carrier Protein B, Ubiquitin-protein Ligase B卵白布局
氨基酸序列
MHHHHHHAMG QLRSMSTPAR RRLMRDFKRL QEDPPVGVSG APSENNIMQW NAVIFGPEGT PFEDGTFKLV IEFSEEYPNK PPTVRFLSKM FHPNVYADGS ICLDILQNRW SPTYDVSSIL TSIQSLLDEP NPNSPANSQA AQLYQENKRE YEKRVSAIVE QSWNDSAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 19.0 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids of human UBE2B and 14 a.a. vector sequence including 6 × His tag at N-terminus.生物活性
No data available.表面
Sterile Colorless liquid.配方
A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.6 with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.内毒素
Less than 1 EU/ug of rHuUBE2B, His as determined by LAL method.消融申明
贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.参考材料
1. Roest HP, van Klaveren J, de Wit J, et al. 1996. Cell, 86: 799-810.2. Adegoke OA, Bedard N, Roest HP, et al. 2002. Am J Physiol Endocrinol Metab, 283: E482-9.3. Kavakebi P, Hausott B, Tomasino A, et al. 2005. Mol Cell Neurosci, 29: 559-68.4. Mulugeta Achame E, Wassenaar E, Hoogerbrugge JW, et al. 2010. BMC Genomics, 11: 367.纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
