Recombinant Murine Beta-defensin 1 (rMuBD-1)
英文全名
Recombinant Murine Beta-defensin 1 (rMuBD-1)英文又名
Defensin beta1卵白布局
氨基酸序列
VGILTSLGRR TDQYKCLQHG GFCLRSSCPS NTKLQGTCKP DKPNCCKSAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 5.2 kDa, a single non-glycosylated polypeptide chain containing 48 amino acids.生物活性
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100.0-1000.0 ng/ml.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.内毒素
Less than 1 EU/ug of rMuBD-1 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Wang YS, Wang GQ, Wen YJ, et al. 2007. Clin Cancer Res, 13: 6779-87.2. Morrison GM, Davidson DJ, Kilanowski FM, et al. 1998. Mamm Genome, 9: 453-7.3. Huttner KM, Kozak CA, Bevins CL. 1997. FEBS Lett, 413: 45-9.4. Yudin AI, Tollner TL, Treece CA, et al. 2008. Reproduction, 136: 753-65.纯度
> 97 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
