Recombinant Murine Breast and Kindey-expressed Chemokine/CXCL14 (rMuBRAK/CXCL14)
英文全名
Recombinant Murine Breast and Kindey-expressed Chemokine/CXCL14 (rMuBRAK/CXCL14)英文又名
B-cell and Monocyte-activating Chemokine, Chemokine BRAK, Kidney-expressed Chemokine CXC, MIP-2G, Small-inducible Cytokine B14卵白布局
氨基酸序列
SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIVTTKS MSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEEAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 9.4 kDa, a single non-glycosylated polypeptide chain containing 77amino acids.生物活性
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 400 mM NaCl, pH 7.4, 5 % trehalose.内毒素
Less than 1 EU/ug of rMuBRAK/CXCL14 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Hromas R, Broxmeyer HE, Kim C, et al. 1999. Biochem Biophys Res Commun. 255:703-6.2. Starnes T, Rasila KK, Robertson MJ, et al. 2006. Exp Hematol. 34:1101-5.3. Shurin GV, Ferris RL, Tourkova IL, et al. 2005. J Immunol. 174:5490-8.4. Kurth I, Willimann K, Schaerli P, et al. 2001. J Exp Med. 194:855-61.5. Hara T, Tanegashima K. 2012. J Biochem. 151:469-76.6. Shellenberger TD, Wang M, Gujrati M, et al. 2004. Cancer Res. 64:8262-70.纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
