Recombinant Murine Interferon-gamma (rMuIFN-gamma)
英文全名
Recombinant Murine Interferon-gamma (rMuIFN-gamma)英文又名
卵白布局
氨基酸序列
HGTVIESLES LNNYFNSSGI DVEEKSLFLD IWRNWQKDGD MKILQSQIIS FYLRLFEVLK DNQAISNNIS VIESHLITTF FSNSKAKKDA FMSIAKFEVN NPQVQRQAFN ELIRVVHQLL PESSLRKRKR SRCAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.生物活性
Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.8 ng/ml, corresponding to a specific activity of > 1.3 × 106 IU/mg.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4, containing 5 % trehalose.内毒素
Less than 1 EU/ug of rMuIFN-gamma as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol, 2. Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55.3. Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56.4. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21.纯度
> 96 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
