Recombinant Murine Interleukin-36 Receptor Antagonist Protein (rMuIL-36RA)
英文全名
Recombinant Murine Interleukin-36 Receptor Antagonist Protein (rMuIL-36RA)英文又名
IL-1HY1, IL-1 delta, IL-1F5, IL-1H3, IL-1L1卵白布局
氨基酸序列
VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAEKVIKGEE ISVVPNRALD ASLSPVILGV QGGSQCLSCG TEKGPILKLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTSPEADQPV RLTQIPEDPA WDAPITDFYF QQCDAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids.生物活性
Measured by its ability to inhibit IL-36gamma induced IL-6 production by human PBMCs.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.内毒素
Less than 1 EU/ug of rMuIL-36RA as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Born TL, Smith DE, Garka KE, et al. 2000. J Biol Chem. 275:41528.2. Dunn EF, Gay NJ, Bristow AF, et al. 2003. Biochemistry. 42:10938-44.3. Barton JL, Herbst R, Bosisio D, et al. 2000. Eur J Immunol. 30:3299-308.4. Debets R, Timans JC, Homey B, et al. 2001. J Immunol. 167:1440-6.5. Towne JE, Renshaw BR, Douangpanya J, et al. 2011. J Biol Chem. 286:42594-602.纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
