Recombinant Murine Monocyte Chemotactic Protein-5/CCL12 (rMuMCP-5/CCL12)
英文全名
Recombinant Murine Monocyte Chemotactic Protein-5/CCL12 (rMuMCP-5/CCL12)英文又名
MCP-1-related Chemokine, Small-inducible Cytokine A12卵白布局
氨基酸序列
GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LGAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 9.3 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids.生物活性
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50 ng/ml.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.内毒素
Less than 1 EU/ug of rMuMCP-5/CCL12 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Sarafi MN, Garcia-Zepeda EA, MacLean JA, et al. 1997. J Exp Med. 185:99-109.2. Jia GQ, Gonzalo JA, Lloyd C, et al. 1996. J Exp Med. 184:1939-51.纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
