Recombinant Murine soluble Tumor Necrosis Factor Receptor Type II/TNFRSF1B (rMusTNFRII/TNFRSF1B)
英文全名
Recombinant Murine soluble Tumor Necrosis Factor Receptor Type II/TNFRSF1B (rMusTNFRII/TNFRSF1B)英文又名
卵白布局
氨基酸序列
VPAQVVLTPY KPEPGYECQI SQEYYDRKAQ MCCAKCPPGQ YVKHFCNKTS DTVCADCEAS MYTQVWNQFR TCLSCSSSCT TDQVEIRACT KQQNRVCACE AGRYCALKTH SGSCRQCMRL SKCGPGFGVA SSRAPNGNVL CKACAPGTFS DTTSSTDVCR PHRICSILAI PGNASTDAVC APESPTLSAI PRTLYVSQPE PTRSQPLDQE PGPSQTPSIL TSLGSTPIIE QSTKGGAccession
Gene ID
产物英文概述
来历
份子量
Approximately 25.3 kDa, a single non-glycosylated polypeptide chain containing 236 amino acids.生物活性
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 cells is less than 2 ug/ml, corresponding to a specific activity of > 500 IU/mg in the presence of 0.1 ng/mL of rMuTNF-alpha.表面
Sterile Filtered White lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.内毒素
Less than 0.1 EU/ug of rMusTNF RII/TNFRSF1B as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
纯度
> 97 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
