Recombinant Rat Cytokine-induced Neutrophil Chemoattractant 1/CXCL1 (rRtCINC-1/CXCL1)
英文全名
Recombinant Rat Cytokine-induced Neutrophil Chemoattractant 1/CXCL1 (rRtCINC-1/CXCL1)英文又名
GRO-alpha, Platelet-derived Growth Factor-inducible Protein KC卵白布局
氨基酸序列
APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PKAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 72 amino acids.生物活性
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.内毒素
Less than 1 EU/ug of rRtCINC-1/CXCL1 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6.2. Richmond A, Thomas HG. 1988. J Cell Biochem. 36:185-98.3. Iida N, Grotendorst GR. 1990. Mol Cell Biol. 10:5596-9.4. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.5. Moser B, Clark-Lewis I, Zwahlen R, et al. 1990. J Exp Med. 171:1797-802.6. Devalaraja RM, Nanney LB, Du J, et al. 2000. J Invest Dermatol. 115:234-44.纯度
> 97 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
