Recombinant Rat gamma-Interferon Inducible Protein 10/CXCL10 (rRtIP-10/CXCL10)
英文全名
Recombinant Rat gamma-Interferon Inducible Protein 10/CXCL10 (rRtIP-10/CXCL10)英文又名
Gamma-IP10, Small-inducible Cytokine B10, Protein Mob-1卵白布局
氨基酸序列
IPLARTVRCT CIDFHEQPLR PRAIGKLEII PASLSCPHVE IIATMKKNNE KRCLNPESEA IKSLLKAVSQ RRSKRAPAccession
Gene ID
产物英文概述
CXCL10 also known as IP-10 is belonging to the CXC chemokine family. It is encoded by the CXCL10 gene, and in rat it is also named the Mob-1 gene. This gene was identified on the basis of differential expression in rat embryo fibroblasts. CXCL10 elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also has other roles, such as promotion of T cell adhesion to endothelial cells, and inhibition of bone marrow colony formation and angiogenesis. Rat CXCL10 shares approximately 68 % amino acid sequence identity with human CXCL10.来历
Escherichia coli.份子量
Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids.生物活性
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR3 transfected HEK293 cells is in a concentration range of 10-50 ng/ml.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.内毒素
Less than 1 EU/ug of rRtIP-10/CXCL10 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
