Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor (rRtGM-CSF)
英文全名
Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor (rRtGM-CSF)英文又名
Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α卵白布局
氨基酸序列
APTRSPNPVT RPWKHVDAIK EALSLLNDMR ALENEKNEDV DIISNEFSIQ RPTCVQTRLK LYKQGLRGNL TKLNGALTMI ASHYQTNCPP TPETDCEIEV TTFEDFIKNL KGFLFDIPFD CWKPVQKAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 14.5 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.生物活性
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine FDC-P1 is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.内毒素
Less than 1 EU/ug of rRtGM-CSF as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3.2. 1989. N Engl J Med, 320: 253-4.3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101.4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27.5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.纯度
> 98 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
