Recombinant Rat Interleukin-33 (rRtIL-33)
英文全名
Recombinant Rat Interleukin-33 (rRtIL-33)英文又名
IL-33卵白布局
氨基酸序列
SIQGTSLLTE SCALSTYNDQ SVSFVLENGC YVINVEDCGK NQEKDKVLLR YYESSFPAQS GDGVDGKKLM VNMSPIKDTD IWLNANDKDY SVELQKGDVS PPDQAFFVLH KKSSDFVSFE CKNLPGTYIG VKDNQLALVE ENDESCNNIM FKLSKMAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.生物活性
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.5.内毒素
Less than 1 EU/ug of rRtIL-33 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Lingel A, Weiss TM, Niebuhr M, et al. 2009. Structure. 17:1398-410.2. Baekkevold ES, Roussigne M, Yamanaka T, et al. 2003. Am J Pathol. 163:69-79.3. Onda H, Kasuya H, Takakura K, et al. 1999. J Cereb Blood Flow Metab. 19:1279-88.4. Black RA, Kronheim SR, Merriam JE, et al. 1989. J Biol Chem. 264:5323-6.纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
