Recombinant Rat Macrophage Inflammatory Protein-1 beta/CCL4 (rRtMIP-1beta/CCL4)
英文全名
Recombinant Rat Macrophage Inflammatory Protein-1 beta/CCL4 (rRtMIP-1beta/CCL4)英文又名
Small-inducible Cytokine A4卵白布局
氨基酸序列
APIGSDPPTS CCFSYTSRKI HRNFVMDYYE TSSLCSQPAV VFLTKKGRQI CADPSEPWVN EYVNDLELNAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.生物活性
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-1000 ng/ml.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4, 3 % trehalose.内毒素
Less than 1 EU/ug of rRtMIP-1β/CCL4 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9.2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31.3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94.纯度
> 95 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
