Recombinant Rat Stromal-Cell Derived Factor-1 beta/CXCL12 beta (rRtSDF-1beta/CXCL12beta)
英文全名
Recombinant Rat Stromal-Cell Derived Factor-1 beta/CXCL12 beta (rRtSDF-1beta/CXCL12beta)英文又名
卵白布局
氨基酸序列
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNKRL KMAccession
Gene ID
产物英文概述
来历
份子量
Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids.生物活性
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 100-200 ng/ml.表面
Sterile Filtered White lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.内毒素
Less than 1 EU/ug of rRtSDF-1beta/CXCL12beta as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500.2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9.3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51.4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9.5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23.6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703.7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53.纯度
> 97 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
