Recombinant Viral Macrophage Inflammatory Protein-2 (rViMIP-2)
英文全名
Recombinant Viral Macrophage Inflammatory Protein-2 (rViMIP-2)英文又名
Viral Macrophage Inflammatory Protein II, vMIP-1B, MIP-II, vMIP-2卵白布局
氨基酸序列
LGASWHRPDK CCLGYQKRPL PQVLLSSWYP TSQLCSKPGV IFLTKRGRQV CADKSKDWVK KLMQQLPVTAAccession
Gene ID
产物英文概述
来历
Escherichia coli.份子量
Approximately 8.0 kDa, a single, non-glycosylated polypeptide chain containing 70 amino acids.生物活性
Fully biologically active when compared to standard. The specific activity is determined by the inhibitory effect on monocyte migration response to human MIP-1 alpha using a concentration range of 1.0ug-10.0ug/ml of viral MIP-2 will inhibit 25ng/ml of human MIP-1 alpha.表面
Sterile filtered white lyophilized (freeze-dried) powder.配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150mM NaCl.内毒素
Less than 1 EU/ug of rViMIP-2 as determined by LAL method.消融申明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.贮存前提
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.参考材料
1. Liwang AC, Wang ZX, Sun Y, et al. 1999. Protein Sci, 8: 2270-80.2. Morris KV, Higgins J, Shen X, et al. 2003. Virus Res, 94: 103-12.3. Luttichau HR. 2008. Virol J, 5: 50.4. Shao W, Fernandez E, Sachpatzidis A, et al. 2001. Eur J Biochem, 268: 2948-59.纯度
> 97 % by SDS-PAGE and HPLC analyses.SDS-PAGE电泳
