The protein has a predicted MW of 25.7 kDa. Due to glycosylation, the protein migrates to 26-30 kDa based on Tris-Bis PAGE result.
活性
Immobilized Rhesus macaque Complement Factor D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 16.3ng/ml determined by ELISA. See testing image for detail.
产物标签
C-His
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in 50mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.