Recombinant SARS-CoV-2 Spike RBD (Omicron XBB.1.5) Protein is expressed from HEK293 with His tag at the C-terminal.It contains Arg319-Phe541(G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H).
The protein has a predicted MW of 26.22 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
活性
Immobilized SARS-Cov-2 Spike RBD (Omicron XBB.1.5) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 19.1ng/ml determined by ELISA. See testing image for detail.
产物标签
C-His
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Liquid
运输前提
Shipped with blue ice.
制剂
Supplied as 0.22um filtered solution in PBS (pH 7.4).
复溶
存储和不变性
Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.