Recombinant SARS-COV-2 Spike S1 (N501Y) Protein is expressed from HEK293 with His tag at the C-terminal.It contains Val16-Arg685.
Biotinylated
产物规格
靶点
Spike S1 (N501Y)
份子又名
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
Accession
种属
SARS-COV-2
卵白序列
Val16-Arg685
份子量
The protein has a predicted MW of 76 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
活性
Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 66.2ng/ml determined by ELISA. See testing image for detail.
产物标签
C-His
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.