Recombinant SARS spike S1 protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminal.It contains Ser14-Arg667.
Biotinylated
产物规格
靶点
Spike S1
份子又名
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
Accession
种属
SARS
卵白序列
Ser14-Arg667
份子量
The protein has a predicted MW of 100.6 kDa. Due to glycosylation, the protein migrates to 120-140 kDa based on Tris-Bis PAGE result.
活性
Immobilized Human ACE2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for SARS Spike S1, hFc Tag with the EC50 of 1.7ug/ml determined by ELISA. See testing image for detail.
产物标签
C-hFc-Avi
抒发体系
HEK293
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per ug by the LAL method.
运输及存储
产物表面
Lyophilized
运输前提
Shipped at ambient temperature.
制剂
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储和不变性
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.