T7 RNA Polymerase is the key enzyme for in vitro transcription of mRNA. It efficiently incorporates standard or modified nucleoside triphosphates (NTPs) into RNA transcripts. T7 RNA Polymerase initiates transcription at the G* in the classic T7 promoter-taatacgactcactataG*GG. When using the GAG cap1-Analog for co-transcription, taatacgactcactataA*GG is recommended.
Note: The cofactor Mg2+ is required for the synthesis. Restriction enzymes that produce blunt ends or 5′-overhangs are recommended.
This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #037660.
Biotinylated
产物规格
靶点
份子又名
Accession
种属
卵白序列
份子量
98 kD
活性
产物标签
抒发体系
纯度
内毒素
运输及存储
产物表面
Liquid
运输前提
Shipped with blue ice
制剂
复溶
存储和不变性
-20±5°C for 24 months. Avoid repeated freeze-thaw cycles.