The 7-methylguanosine cap structure (m7Gppp, Cap0) is a specific feature in eukaryotic mRNA, which is required for mRNA stability, splicing, nuclear export, effective translation, and mRNA decay. Vaccinia Capping Enzyme, a heterodimer of D1 and D12 subunits, adding the Cap0 structure to the 5′ end of the RNA. The D1 subunit comprises an N-terminal RNA triphosphatase (TPase), a guanylyltransferase (GTase), and a C-terminal guanine-N7-methyltransferase (MTase). The D12 subunit binds and stimulates the MTase.
NOTE: mRNA Cap 2′-O-Methyltransferase (ChemWhat #GMP-MEH-VE101) is required to generate Cap 1 structure that reduces cellular innate immune response when the RNA is used in vivo .
This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #038028.
Biotinylated
产物规格
靶点
份子又名
Accession
种属
卵白序列
份子量
活性
产物标签
抒发体系
纯度
内毒素
运输及存储
产物表面
Liquid
运输前提
Shipped with blue ice
制剂
复溶
存储和不变性
-20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
RNase III, Accession: